Publications from the Ehrlicher lab

Nuclear curvature determines Yes-associated protein localization and differentiation of mesenchymal stem cells

Ajinkya Ghagre, Alice Delarue, Luv Kishore Srivastava, Newsha Koushki, Allen Ehrlicher

Biophysical Journal, 2024, VOLUME 123, ISSUE 10, P1222-1239, MAY 21, 2024, Published:April 10, 2024DOI:https://doi.org/10.1016/j.bpj.2024.04.008

Nuclear compression regulates YAP spatiotemporal fluctuations in living cells.

Koushki N, Ghagre A, Srivastava LK, Molter C, Ehrlicher AJ.

Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2301285120. doi: 10.1073/pnas.2301285120.

Centrifugation and index matching yield a strong and transparent bioinspired nacreous composite.

Amini A, Khavari A, Barthelat F, Ehrlicher AJ.

Science. 2021 Sep 10;373(6560): 1229-1234. doi:10.1126/science.abf0277.

Pattern-Based Contractility Screening, a Reference-Free Alternative to Traction Force Microscopy Methodology. Ghagre A, Amini A, Srivastava LK, Tirgar P, Khavari A, Koushki N, Ehrlicher A.

ACS Appl Mater Interfaces. 2021 May 5;13(17):19726-19735. doi: 10.1021/acsami.1c02987.

Spatial distribution of lamin A/C determines nuclear stiffness and stress-mediated deformation.

Srivastava LK, Ju Z, Ghagre A, Ehrlicher AJ.

J Cell Sci. 2021 May 15;134(10):jcs248559. doi: 10.1242/jcs.248559.

Traction Force Screening Enabled by Compliant PDMS Elastomers.

Yoshie H, Koushki N, Kaviani R, Tabatabaei M, Rajendran K, Dang Q, Husain A, Yao S, Li C, Sullivan JK, Saint-Geniez M, Krishnan R, Ehrlicher AJ.

Biophys J. 2018 May 8;114(9):2194-2199. doi: 10.1016/j.bpj.2018.02.045.

Probing the stochastic, motor-driven properties of the cytoplasm using force spectrum microscopy.

Guo M, Ehrlicher AJ, Jensen MH, Renz M, Moore JR, Goldman RD, Lippincott-Schwartz J, Mackintosh FC, Weitz DA.

Cell. 2014 Aug 14;158(4):822-832. doi: 10.1016/j.cell.2014.06.051.

Mechanical strain in actin networks regulates FilGAP and integrin binding to filamin A.

Ehrlicher AJ, Nakamura F, Hartwig JH, Weitz DA, Stossel TP.

Nature. 2011 Sep 18;478(7368):260-3. doi: 10.1038/nature10430.

Endocytic proteins with prion-like domains form viscoelastic condensates that enable membrane remodeling.

Bergeron-Sandoval LP, Kumar S, Heris HK, Chang CLA, Cornell CE, Keller SL, François P, Hendricks AG, Ehrlicher AJ, Pappu RV, Michnick SW. Proc Natl Acad Sci U S A. 2021 Dec 14;118(50):e2113789118. doi: 10.1073/pnas.2113789118.

Advancing the Mechanical Performance of Glasses: Perspectives and Challenges

Wondraczek L, Bouchbinder E, Ehrlicher A, Mauro JC, Sajzew R, Smedskjaer MM. Adv Mater. 2021 Dec 6:e2109029. doi: 10.1002/adma.202109029.

Centrifugation and index matching yield a strong and transparent bioinspired nacreous composite.

Amini A, Khavari A, Barthelat F, Ehrlicher AJ.

Science. 2021 Sep 10;373(6560): 1229-1234. doi:10.1126/science.abf0277.

Pattern-Based Contractility Screening, a Reference-Free Alternative to Traction Force Microscopy Methodology. Ghagre A, Amini A, Srivastava LK, Tirgar P, Khavari A, Koushki N, Ehrlicher A.

ACS Appl Mater Interfaces. 2021 May 5;13(17):19726-19735. doi: 10.1021/acsami.1c02987.

Spatial distribution of lamin A/C determines nuclear stiffness and stress-mediated deformation.

Srivastava LK, Ju Z, Ghagre A, Ehrlicher AJ.

J Cell Sci. 2021 May 15;134(10):jcs248559. doi: 10.1242/jcs.248559.

Bioprintable, Stiffness-Tunable Collagen-Alginate Microgels for Increased Throughput 3D Cell Culture Studies.

Ort C, Chen Y, Ghagre A, Ehrlicher A, Moraes C.

ACS Biomater Sci Eng. 2021 Jun 14;7(6):2814-2822. doi: 10.1021/acsbiomaterials.1c00129.

Toward embryo cryopreservation-on-a-chip: A standalone microfluidic platform for gradual loading of cryoprotectants to minimize cryoinjuries.

Tirgar P, Sarmadi F, Najafi M, Kazemi P, AzizMohseni S, Fayazi S, Zandi G, Ziaie N, Shoushtari Zadeh Naseri A, Ehrlicher A, Dashtizad M. Biomicrofluidics. 2021 May 18;15(3):034104. doi: 10.1063/5.0047185.

A nondestructive contactless technique to assess the viscoelasticity of blood clots in real-time.

Naseri S, Koushki N, Rezabeigi E, Ehrlicher A, Nazhat SN.

J Mech Behav Biomed Mater. 2020 Oct;110:103921. doi: 10.1016/j.jmbbm.2020.103921.

Dynamic actin cross-linking governs the cytoplasm's transition to fluid-like behavior.

Chaubet L, Chaudhary AR, Heris HK, Ehrlicher AJ, Hendricks AG.

Mol Biol Cell. 2020 Jul 21;31(16):1744-1752. doi: 10.1091/mbc.E19-09-0504.

Tissue traction microscopy to quantify muscle contraction within precision-cut lung slices.

Ram-Mohan S, Bai Y, Schaible N, Ehrlicher AJ, Cook DP, Suki B, Stoltz DA, Solway J, Ai X, Krishnan R.

Am J Physiol Lung Cell Mol Physiol. 2020 Feb 1;318(2):L323-L330. doi: 10.1152/ajplung.00297.2019.

Nuclei deformation reveals pressure distributions in 3D cell clusters.

Khavari A, Ehrlicher AJ.

PLoS One. 2019 Sep 12;14(9):e0221753. doi: 10.1371/journal.pone.0221753.

Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics.

Jiang T, Munguia-Lopez JG, Gu K, Bavoux MM, Flores-Torres S, Kort-Mascort J, Grant J, Vijayakumar S, De Leon-Rodriguez A, Ehrlicher AJ, Kinsella JM.

Biofabrication. 2019 Dec 31;12(1):015024. doi: 10.1088/1758-5090/ab3a5c.

High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition.

Yoshie H, Koushki N, Molter C, Siegel PM, Krishnan R, Ehrlicher AJ.

J Vis Exp. 2019 Jun 1;(148):10.3791/59364. doi: 10.3791/59364.

Multiplexed, high-throughput measurements of cell contraction and endothelial barrier function.

Rokhzan R, Ghosh CC, Schaible N, Notbohm J, Yoshie H, Ehrlicher AJ, Higgins SJ, Zhang R, Haller H, Hardin CC, David S, Parikh SM, Krishnan R.

Lab Invest. 2019 Jan;99(1):138-145. doi: 10.1038/s41374-018-0136-2.

Neuronal and metastatic cancer cells: Unlike brothers.

Heine P, Ehrlicher A, Käs J.

Biochim Biophys Acta. 2015 Nov;1853(11 Pt B):3126-31. doi: 10.1016/j.bbamcr.2015.06.011.

Alpha-actinin binding kinetics modulate cellular dynamics and force generation.

Ehrlicher AJ, Krishnan R, Guo M, Bidan CM, Weitz DA, Pollak MR.

Proc Natl Acad Sci U S A. 2015 May 26;112(21):6619-24. doi: 10.1073/pnas.1505652112.

Structurally governed cell mechanotransduction through multiscale modeling.

Kang J, Puskar KM, Ehrlicher AJ, LeDuc PR, Schwartz RS.

Sci Rep. 2015 Feb 27;5:8622. doi: 10.1038/srep08622.

Microtubule sliding drives proplatelet elongation and is dependent on cytoplasmic dynein.

Bender M, Thon JN, Ehrlicher AJ, Wu S, Mazutis L, Deschmann E, Sola-Visner M, Italiano JE, Hartwig JH.

Blood. 2015 Jan 29;125(5):860-8. doi: 10.1182/blood-2014-09-600858.

Platelet bioreactor-on-a-chip.

Thon JN, Mazutis L, Wu S, Sylman JL, Ehrlicher A, Machlus KR, Feng Q, Lu S, Lanza R, Neeves KB, Weitz DA, Italiano JE Jr.

Blood. 2014 Sep 18;124(12):1857-67. doi: 10.1182/blood-2014-05-574913.

Probing the stochastic, motor-driven properties of the cytoplasm using force spectrum microscopy.

Guo M, Ehrlicher AJ, Jensen MH, Renz M, Moore JR, Goldman RD, Lippincott-Schwartz J, Mackintosh FC, Weitz DA.

Cell. 2014 Aug 14;158(4):822-832. doi: 10.1016/j.cell.2014.06.051.

The role of vimentin intermediate filaments in cortical and cytoplasmic mechanics.

Guo M, Ehrlicher AJ, Mahammad S, Fabich H, Jensen MH, Moore JR, Fredberg JJ, Goldman RD, Weitz DA.

Biophys J. 2013 Oct 1;105(7):1562-8. doi: 10.1016/j.bpj.2013.08.037.

Microwave dielectric heating of non-aqueous droplets in a microfluidic device for nanoparticle synthesis.

Koziej D, Floryan C, Sperling RA, Ehrlicher AJ, Issadore D, Westervelt R, Weitz DA.

Nanoscale. 2013 Jun 21;5(12):5468-75. doi: 10.1039/c3nr00500c.

Controlled synthesis of cell-laden microgels by radical-free gelation in droplet microfluidics.

Rossow T, Heyman JA, Ehrlicher AJ, Langhoff A, Weitz DA, Haag R, Seiffert S.

J Am Chem Soc. 2012 Mar 14;134(10):4983-9. doi: 10.1021/ja300460p.

Actin filament elasticity and retrograde flow shape the force-velocity relation of motile cells.

Zimmermann J, Brunner C, Enculescu M, Goegler M, Ehrlicher A, Käs J, Falcke M.

Biophys J. 2012 Jan 18;102(2):287-95. doi: 10.1016/j.bpj.2011.12.023.

Mechanical strain in actin networks regulates FilGAP and integrin binding to filamin A.

Ehrlicher AJ, Nakamura F, Hartwig JH, Weitz DA, Stossel TP.

Nature. 2011 Sep 18;478(7368):260-3. doi: 10.1038/nature10430.

Dewetting-induced membrane formation by adhesion of amphiphile-laden interfaces.

Shum HC, Santanach-Carreras E, Kim JW, Ehrlicher A, Bibette J, Weitz DA.

J Am Chem Soc. 2011 Mar 30;133(12):4420-6. doi: 10.1021/ja108673h.

Cell mechanics: Contracting to stiffness.

Ehrlicher A, Hartwig JH.

Nat Mater. 2011 Jan;10(1):12-3. doi: 10.1038/nmat2928.

Cytoskeletal mechanics of proplatelet maturation and platelet release.

Thon JN, Montalvo A, Patel-Hett S, Devine MT, Richardson JL, Ehrlicher A, Larson MK, Hoffmeister K, Hartwig JH, Italiano JE Jr.

J Cell Biol. 2010 Nov 15;191(4):861-74. doi: 10.1083/jcb.201006102.

Buckling, stiffening, and negative dissipation in the dynamics of a biopolymer in an active medium.

Kikuchi N, Ehrlicher A, Koch D, Käs JA, Ramaswamy S, Rao M.

Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19776-9. doi: 10.1073/pnas.0900451106.

Optical neuronal guidance.

Ehrlicher A, Betz T, Stuhrmann B, Gögler M, Koch D, Franze K, Lu Y, Käs J.

Methods Cell Biol. 2007;83:495-520. doi: 10.1016/S0091-679X(07)83021-4.

The forces behind cell movement.

Ananthakrishnan R, Ehrlicher A.

Int J Biol Sci. 2007 Jun 1;3(5):303-17. doi: 10.7150/ijbs.3.303.

Cell migration through small gaps.

Brunner CA, Ehrlicher A, Kohlstrunk B, Knebel D, Käs JA, Goegler M.

Eur Biophys J. 2006 Oct;35(8):713-9. doi: 10.1007/s00249-006-0079-1.

Guiding neuronal growth with light.

Ehrlicher A, Betz T, Stuhrmann B, Koch D, Milner V, Raizen MG, Kas J.

Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16024-8. doi: 10.1073/pnas.252631899.

McGill University is located on land which has long served as a site of meeting and exchange amongst Indigenous peoples. We honor, recognize, and respect these nations as the traditional stewards of the lands and waters on which we meet today.

Dr. Allen Ehrlicher

Department of Bioengineering

McConnell Engineering Building

3480 University Street, Room 350

Montreal, Quebec H3A 2A7

Phone: 514-714-8239

Fax: 514-398-7379

Email: allen.ehrlicher@mcgill.ca

Office: McConnell Engineering Building 358